Janus激酶3(JAK3)活性蛋白
Active Janus Kinase 3 (JAK3)
JAKL; L-JAK; LJAK; A Protein Tyrosine Kinase,Leukocyte; Leukocyte janus kinase; Tyrosine-protein kinase JAK3
- 编号APF493Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 97%
- 等电点8.5
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 2160 ¥ 5400 ¥ 10800 ¥ 32400 ¥ 81000
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产品包装(模拟)
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产品包装(模拟)
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Gene sequencing
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SDS-PAGE图
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Figure. Western Blot; Sample: Recombinant JAK3, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
JAK3 (Tyrosine-protein kinase JAK3) is a non-receptor tyrosine kinase that involved in various processes such as cell growth, development, or differentiation. Besides, STAM2 has been identified as an interactor of JAK3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK3 and recombinant human STAM2. Briefly, JAK3 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to STAM2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-JAK3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of JAK3 and STAM2 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPF493Hu01 | Janus激酶3(JAK3)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APF493Hu01 | Janus激酶3(JAK3)活性蛋白 | Cell culture; Activity Assays. |
| PAF493Hu01 | Janus激酶3(JAK3)多克隆抗体 | WB; IHC; ICC; IP. |
| MAF493Hu21 | Janus激酶3(JAK3)单克隆抗体 | WB; IHC; ICC; IP. |
| SEF493Hu | Janus激酶3(JAK3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF493Hu | Janus激酶3(JAK3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |