突触融合蛋白1A(STX1A)活性蛋白
Active Syntaxin 1A, Brain (STX1A)
STX-1A; HPC-1; STX1; P35-1; Neuron-specific antigen HPC-1
- 编号APD035Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 90%
- 等电点4.6
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
活性实验
Syntaxin 1A (STX1A), a member of the syntaxin family ,is a nervous system-specific membrane protein responsible for fusion.Synaptosomal Associated Protein 25kDa (SNAP25) binds to STX1A to form the SNARE complex , which is essential for the release of neurotransmitters.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human STX1A and recombinant human SNAP25. Briefly, STX1A was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to SNAP25-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-STX1A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human STX1A and recombinant human SNAP25 was shown in Figure 1, the EC50 for this effect is 0.026ug/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD035Hu01 | 突触融合蛋白1A(STX1A)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD035Hu01 | 突触融合蛋白1A(STX1A)活性蛋白 | Cell culture; Activity Assays. |
| PAD035Hu01 | 突触融合蛋白1A(STX1A)多克隆抗体 | WB; IHC; ICC; IP. |
| MAD035Hu21 | 突触融合蛋白1A(STX1A)单克隆抗体 | WB; IHC; ICC; IP. |
| SED035Hu | 突触融合蛋白1A(STX1A)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD035Hu | 突触融合蛋白1A(STX1A)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |